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. 2020 Apr 20;295(22):7554–7565. doi: 10.1074/jbc.RA120.013102

Table 1.

Plasmids used in this study

Plasmid Base plasmid/Genotype Source
pRS315-5FLAG-SCC1 ampr/LEU2 5FLAG-SCC1 This study
pPADH1-5FLAG-SCC1 ampr/HIS3 5FLAG-SCC1 This study
pPADH1-5FLAG-SCC3 ampr/LEU2 5FLAG-SCC3 This study
pPADH1-13MYC-SCC3 ampr/HIS3 13MYC-SCC3 This study
pPADH1-5FLAG-scc3 K99C ampr/HIS3 5FLAG-scc3 K99C This study
pPADH1-5FLAG-scc3 K764C ampr/HIS3 5FLAG-scc3 K764C This study
pPADH1-13MYC-scc3 K1076C ampr/LEU2 13MYC-scc3 K1076C This study
pPADH1-13MYC-scc3 K1057C ampr/LEU2 13MYC-scc3 K1057C This study
pRS313-ECO1 ampr/HIS3 ECO1 This study
pRS313-PCUP1-td-ECO1-13MYC-aid ampr/HIS3 td-ECO1–13MYC-aid This study
pRK5-FLAG-SMC3 ampr/FLAG-SMC3 This study
pRK5-MYC-SMC3 ampr/MYC-SMC3 This study
pGEX-6P-1-SCC1 ampr/GST-SCC1 This study
pGEX-6P-1-SCC3 ampr/GST-SCC3 This study
pET28a-SCC1 kanr/His6-SCC1 This study
pET28a-SCC3 kanr/His6-SCC3 This study