Table 1.
Plasmid | Base plasmid/Genotype | Source |
---|---|---|
pRS315-5FLAG-SCC1 | ampr/LEU2 5FLAG-SCC1 | This study |
pPADH1-5FLAG-SCC1 | ampr/HIS3 5FLAG-SCC1 | This study |
pPADH1-5FLAG-SCC3 | ampr/LEU2 5FLAG-SCC3 | This study |
pPADH1-13MYC-SCC3 | ampr/HIS3 13MYC-SCC3 | This study |
pPADH1-5FLAG-scc3 K99C | ampr/HIS3 5FLAG-scc3 K99C | This study |
pPADH1-5FLAG-scc3 K764C | ampr/HIS3 5FLAG-scc3 K764C | This study |
pPADH1-13MYC-scc3 K1076C | ampr/LEU2 13MYC-scc3 K1076C | This study |
pPADH1-13MYC-scc3 K1057C | ampr/LEU2 13MYC-scc3 K1057C | This study |
pRS313-ECO1 | ampr/HIS3 ECO1 | This study |
pRS313-PCUP1-td-ECO1-13MYC-aid | ampr/HIS3 td-ECO1–13MYC-aid | This study |
pRK5-FLAG-SMC3 | ampr/FLAG-SMC3 | This study |
pRK5-MYC-SMC3 | ampr/MYC-SMC3 | This study |
pGEX-6P-1-SCC1 | ampr/GST-SCC1 | This study |
pGEX-6P-1-SCC3 | ampr/GST-SCC3 | This study |
pET28a-SCC1 | kanr/His6-SCC1 | This study |
pET28a-SCC3 | kanr/His6-SCC3 | This study |