Table 2.
Strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 lys2Δ0 | In stock |
| BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | In stock |
| YSD03 | BY4741 KanMX6::PGAL1-GFP-SCC1 (p315-5FLAG-SCC1::LEU2) | This study (Fig. 1B) |
| YSD24 | BY4741 SCC1-3HA-EPEA::HygR (p315-5FLAG-SCC1::LEU2) | This study (Fig. 1A) |
| YSD107 | BY4743 5FLAG-SCC1::HIS3/GFP-SCC1::LEU2 | This study (Fig. 1C) |
| ZSJ22 | BY4741 KanMX6::PADH1-GST-SCC1 wpl1Δ::HygR (p315-5FLAG-SCC1::LEU2) | This study (Fig. 2A) |
| YSD61 | BY4741 KanMX6::PGAL1-GFP-SCC3 (pPADH1-5FLAG-SCC3::LEU2) | This study (Fig. 1E) |
| YSD109 | BY4743 5FLAG-SCC3::HIS3/GFP-SCC3::LEU2 | This study (Fig. 1D) |
| YSD83 | BY4741 scc3Δ::NatMX (pScc3::URA3 pPADH1-5FLAG-SCC3::HIS3 pPADH1-13MYC-SCC3::LEU2) | This study (Fig. 3, C and D) |
| YSD87 | BY4741 scc3Δ::NatMX (pScc3::URA3 pPADH1-5FLAG- scc3 K99C::HIS3 pPADH1-13MYC-scc3 K1057C::LEU2) | This study (Fig. 3, C and D) |
| YSD88 | BY4741 scc3Δ::NatMX (pScc3::URA3 pPADH1-5FLAG- scc3 K764C::HIS3 pPADH1-13MYC-scc3 K1076C::LEU2) | This study (Fig. 3, C and D) |
| YSD17 | BY4741 KanMX6::PGAL1-GFP-SCC1 wpl1Δ::HygR (p315-5FLAG-SCC1::LEU2) | This study (Fig. 5, A and C) |
| YSD08 | BY4741 KanMX6::PGAL1-GFP-SCC1 hos1Δ::HygR (p315-5FLAG-SCC1::LEU2) | This study (Fig. 5, A and C) |
| YSD159 | BY4743 5FLAG-SCC1::HIS3/GFP-SCC1::LEU2 hos1Δ::HygR/hos1Δ::NatMX | This study (Fig. 5B) |
| YSD141 | BY4743 5FLAG-SCC1::HIS3 / GFP-SCC1::LEU2 wpl1Δ::HygR/wpl1Δ::NatMX | This study (Fig. 5D) |
| YSD33 | BY4741 SCC1-3HA-EPEA::HygR eco1Δ::NatMX ubr1::PGal1-UBR1-PGAP-OsTIR1-9MYC-URA3 (p315-5FLAG-SCC1::LEU2 p313-ECO1::HIS3) | This study (Fig. 3, E–H) |
| YSD35 | BY4741 SCC1-3HA-EPEA::HygR eco1Δ::NatMX ubr1::PGal1-UBR1-PGAP-OsTIR1-9MYC-URA3 (p315-5FLAG-SCC1::LEU2 p313-PCUP1-td-ECO1-13MYC-aid::HIS3) | This study (Fig. 3, E–H) |