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Identification of the ω site in human PrPC from KI mouse brains. A, peptide coverage of purified human PrPC from KI mouse brains. Trypsinized peptides were analyzed by LC-ESI-MS, and the obtained data were analyzed using the MASCOT database. B, alignment of PrPC sequences. Sequence alignment was performed using ClustalW and BoxShade. Residues conserved among species are shaded in gray or black. Red boxes indicate the ω sites determined in this and previous studies (28).
