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. 2020 Apr 22;295(22):7789–7798. doi: 10.1074/jbc.RA120.013444

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© 2020 Kobayashi et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — Determination of GPI glycan structures in human PrP^C from KI mice by LC-ESI-MS/MS analysis. A, left panel, the MS/MS spectra of the m/z 788.72²⁺ ion shown in Fig. S1B and the identities of fragment ions. Right panel, the corresponding structure (MW 1576.2) of a C-terminal peptide with GPI containing a HexNAc-Hex side chain. B, left panel, the MS/MS spectra of the m/z 870.25²⁺ ion. Right panel, the corresponding structure (MW 1738.3) of a C-terminal peptide with GPI containing Hex and HexNAc-Hex side chains. C, left panel, the MS/MS spectra of the m/z 934.3²⁺ ion. Right panel, the corresponding structure (MW 1867.4) of a C-terminal peptide with GPI containing a HexNAc-Hex-Neu5Ac side chain. In A–C, all annotated fragment ions are monovalent cations. D, percentages of GPI glycan species in KI mouse brains. Core only, Core + Hex (fourth Man), and Core + HexNAc (GalNAc) were not detected.