Table 1.

Echocardiographic parameters of WT and transgenic (MANF KD) mice

FS, fractional shortening; EF, ejection fraction; LVEDV, left ventricular end diastolic volume; LVESV, left ventricular end systolic volume; LVIDD, left ventricular inner diameter in diastole; LVIDS, left ventricular inner diameter in systole; PWTD, left ventricular posterior wall thickness in diastole; PWTS, left ventricular posterior wall thickness in systole; AWTD, left ventricular anterior wall thickness in diastole; AWTS, left ventricular anterior wall thickness in systole; LV mass, left ventricular mass; HR, heart rate in beats per minute (bpm). Statistical analyses used Student's unpaired t test. *, p ≤ 0.05, difference between WT and transgenic MANF KD mice of the same sex.

Parameter	Female WT (n = 6)	Female MANF KD (n = 7)	Male WT (n = 6)	Male MANF KD (n = 7)
FS (%)	39.80 ± 3.11	50.47 ± 0.75*	36.89 ± 1.87	45.96 ± 1.72*
EF (%)	70.61 ± 2.72	82.62 ± 0.74*	67.91 ± 2.35	77.82 ± 1.83*
LVEDV (μl)	53.17 ± 2.37	49.82 ± 3.25	41.83 ± 3.02	56.93 ± 3.86*
LVESV (μl)	15.97 ± 2.18	8.74 ± 0.87*	13.18 ± 0.72	12.99 ± 1.94
LVIDD (mm)	3.58 ± 0.09	3.40 ± 0.06	3.22 ± 0.10	3.66 ± 0.10
LVIDS (mm)	2.10 ± 0.14	1.65 ± 0.11*	2.02 ± 0.04	1.98 ± 0.11
PWTD (mm)	0.89 ± 0.05	0.77 ± 0.06	0.92 ± 0.07	1.05 ± 0.14
PWTS (mm)	1.15 ± 0.06	1.32 ± 0.08	1.25 ± 0.12	1.45 ± 0.07
AWTD (mm)	0.85 ± 0.06	0.95 ± 0.11	1.03 ± 0.07	0.94 ± 0.04
AWTS (mm)	1.45 ± 0.07	1.58 ± 0.06	1.43 ± 0.07	1.56 ± 0.08
LV mass (mg)	99.16 ± 8.22	92.65 ± 7.72	106.75 ± 6.94	124.01 ± 14.04
HR (bpm)	472.96 ± 2.98	458.69 ± 5.51	473.88 ± 11.14	453.20 ± 4.58