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. 2020 Apr 21;295(22):7635–7652. doi: 10.1074/jbc.RA120.013336

Figure 2.

Figure 2.

A. finegoldii encodes two functional enoyl-acyl carrier protein reductases. A, growth of temperature-sensitive E. coli strain JP1111 (fabI(Ts)) mutant transformed with empty plasmid vector control (VC), or plasmids expressing either E. coli FabI (EcFabI), C. trachomatis FabI (CtFabI), A. finegoldii FabI (AfFabI; UniProt ID: A0A174E195), C. acetobutylicum FabK (CaFabK), or A. finegoldii FabK (AfFabK; UniProt ID: I3YI65) under the growth-permissive temperature (30 °C). B, growth of the same strain set at the nonpermissive temperature (42 °C). Five biological replicates were tested for each strain. C, microbroth dilution assay to rank the sensitivities of the FabIs and FabKs to AFN-1252 by determining the minimal inhibitory concentrations for AFN-1252 against E. coli strain ANS1 (ΔtolC) (33) transformed with the same series of plasmids described in panel A. Mean ± S.E. (n = 3).