Table 3.
Bacterial strains and plasmids
| Description | Reference | |
|---|---|---|
| Strain | ||
| E. coli JP1111 | Hfr(PO1), galE45(GalS), λ−, fabI392(Ts), relA1, spoT1, thiE1 | 79 |
| E. coli ANS1 | metB1 relA1 spoT1 gyrA216 tolC::Tn10 λ− λr F− | 63 |
| Plasmid | ||
| pPJ131 | E. coli expression vector | 80 |
| pEcFabI | E. coli fabI in pPJ131 | 29 |
| pCtFabI | Chlamydia trachomatis fabI in pPJ131 | 29 |
| pAfFabI | A. finegoldii fabI (UniProt ID: A0A174E195) in pPJ131 | This study |
| pCaFabK | Clostridium acetobutylicum fabK in pPJ131 | 37 |
| pAfFabK | A. finegoldii fabK (UniProt ID: I3YI65) in pPJ131 | This study |
| pET_AfFabI | A. finegoldii fabI 1–289 (UniProt ID: A0A174E195) in pET28b | This study |
| pET_1–273 | A. finegoldii fabI 1–273 (UniProt ID: A0A174E195) in pET28b | This study |
| pET_1–269 | A. finegoldii fabI 1–269 (UniProt ID: A0A174E195) in pET28b | This study |
| pET_1–265 | A. finegoldii fabI 1–265 (UniProt ID: A0A174E195) in pET28b | This study |
| pET_1–261 | A. finegoldii fabI 1–261 (UniProt ID: A0A174E195) in pET28b | This study |