Table II. Numbers of features and other elements in a single LC-IMS-MS/MS run. For a typical run with a 2-hour gradient we show the numbers of scans, detected elements and identified entities.
| Workflow component | Number of items per run in HeLa sample | Number of items per run in plasma sample |
|---|---|---|
| MS1 frames | 7828 | 1729 |
| MS2 frames | 52679 | 3990 |
| IMS scans per frame | 918 | 918 |
| PASEF precursors | 233437 | 44214 |
| 4D features | 2164978 | 540917 |
| 4D isotope patterns | 409379 | 65501 |
| MS/MS submitted | 114477 | 16928 |