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. 2020 May 25;11:874. doi: 10.3389/fmicb.2020.00874

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Copyright © 2020 Batista, Stapleton, Lowther, Fonseca, Shaw, Pond, Walker, van Aerle and Martinez-Urtaza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Amplicons obtained with the primers VP1F and VP1R that amplify a 498-bp fragment of the VP1 region of HAV (Lanes 1 and 2), and amplicons obtained with the primers NH₂ and VP3-1431B that amplify a 455-bp fragment of the VP3 region of HAV (Lanes 3 and 4). Lanes N, negative controls. Lanes L, 100 bp DNA ladder (Promega).