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. 2020 May 25;11:647. doi: 10.3389/fpls.2020.00647

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Copyright © 2020 Allen and Allen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Potting mix Relative Water Content (RWC) and total (undissociated and dissociated) acetic acid ([HAc] + [Ac^–]) treatment impacts in the outdoor Water Use Efficiency (WUE) assay on a range of seedling growth phenotypes (means ± 1 standard error of the mean), including shoot weight (A,B) and Water Content (WC) (G,H), leaf area (K,L) and chlorophyll content (M,N), stem volume (I,J), root area (O,P) and plant transpiration (C,D), WUE (E,F) and developmental stage (Q,R). None of the water by acetic acid interactions were significant (p > 0.1), so only the main effects are presented. Contrasting letters denote significant differences (p < 0.05) within water (A,C,E,G,I,K,M,O,Q) or acetic acid (B,D,F,H,J,L,N,P,R) treatments (blocked factorial ANOVA; Tukey pairwise comparison; n = 6).