Figure 2.

Serum metabolomics analysis of changes in metabolic profiles among control, model and high dose of LHQW groups. Mice were randomly divided into three groups: control group, model group and high dose LHQW group (1,300 mg/kg/day) (n = 10). Model and LHQW groups were infected with H1N1 (2 × LD₅₀ per mouse) 2 h after the first administration and intragastrically administered twice per day (8-hour interval) for five consecutive days. The blood was taken from eyeball after the last oral administration 2 h later, and centrifuged subsequently to obtain supernatant for use. (A) PCA scores plot of comparing control, model and LHQW groups in positive ESI modes (a) and negative ESI modes (b). (B) The OPLS-DA score plots and S-plots generated from the OPLS-DA of the QTOF/MS data from control, model and LHQW groups in the ESI⁻ mode. OPLS-DA score plots were the pair-wise comparisons between the control and the model (a) as well as the model and LHQW (b). S-plot of the OPLS-DA model was for the control and the model (c) and the model and LHQW (d), whose axes plotted in the S-plot from the predictive component are p1 vs. p(corr)1, representing the magnitude (modeled covariation) and reliability (modeled correlation) respectively. (C) Summary of pathway analysis with MetaboAnalyst 3.0 based on the differential metabolites.