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. 2020 May 25;11:753. doi: 10.3389/fphar.2020.00753

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Copyright © 2020 Crentsil, Yamthe, Anibea, Broni, Kwofie, Tetteh and Osei-Safo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flow cytometry analysis of promastigotes following treatment with HA and after labeling with annexin-v and pi (untreated Leishmania major promastigote). (A) Dot Plot analysis of gated L. Major (R1) showing its size (Forward Scatter) and internal granularity (Side Scatter). (B) Dot Plot analysis of cellular apoptotic (Annexin V) and necrotic cells (Propidium Iodide) where L. Major expresses early cellular apoptosis (R2) and necrotic and apoptotic cells (R4). (C) Dot Plot analysis of L. Major being expressed in size (Forward Scatter) and cellular apoptosis (Annexin V). (D) Histogram plot analysis of L. Major showing low apoptotic cell expression (M1) and high apoptotic cell expression.