TABLE 2.

Oxford Nanopore library preparation and sequencing statistics. Non-fragmentation and fragmentation techniques were used in sample preparation.

Library	Fragmentation	Total length (Gb)	Totals reads	N50 (bp)	Mean length (bp)	Median length (bp)	Max length (bp)	Mean quality score
1	No Fragmentation	1.26	55,551	40,434	22,617	15,607	194,834	9.1
2	Zymo	5.61	567,514	23,595	9,877	4,522	153,389	9.4
3	Zymo	2.24	133,660	33,394	16,770	9,857	199,575	9.2
4	Covaris, 4,200 RPM	13.04	1,005,270	15,878	11,760	11,017	181,817	8.3
5	Covaris, 3,800 RPM	10.08	854,994	15,277	11,788	11,104	133,274	9.1
6	Covaris, 3,800 RPM	6.94	501,526	20,681	13,760	12,431	164,726	8.9
7	Covaris, 4,200 RPM	8.86	617,385	19,276	14,343	12,932	231,794	9.1
8	Covaris, 4,200 RPM	10.72	1,221,530	12,664	8,778	8,300	149,453	9.1
9	Covaris, 4,000 RPM	6.64	568,017	17,580	11,686	11,115	128,743	8.9
Avg/Total		65.4	5,525,447	22,087	13,487	10,765	170,845	9