TABLE 4.
Assembly and Benchmarking Universal Single Copy Orthologs (BUSCO) statistics for the MaSuRCA, Flye, wtdbg2, Canu and Hi-C scaffolded Canu assemblies.
| Metrics | Assembler |
||||
| MaSuRCA1 | Flye | wtdbg2 | Canu | Canu Hi-C1 | |
| Number of contigs | 2,850 | 3,009 | 4,659 | 3,183 | 1,325 |
| Total size of the assembly (bp) | 914,348,993 | 813,404,858 | 867,633,014 | 964,774,081 | 965,003,581 |
| Longest (bp) | 10,067,120 | 11,408,532 | 7,183,448 | 9,632,068 | 113,540,806 |
| Shortest (bp) | 2,256 | 178 | 2,807 | 1,072 | 603 |
| Mean (bp) | 320,824 | 270,324 | 186,227 | 303,102 | 728,305 |
| Median (bp) | 39,479 | 100,839 | 15,813 | 63,149 | 35,305 |
| N50 (bp) | 1,317,304 | 698,928 | 1,176,597 | 1,114,696 | 98,884,393 |
| L50 | 169 | 282 | 201 | 223 | 5 |
| G+C% | 36.84 | 36.77 | 36.89 | 37.06 | 37.05 |
| BUSCO metrics | |||||
| %Complete COGS found [%single, %duplicate] | 90.0% [85.8%, 4.2%] | 90.6% [88.1%, 2.5%] | 86.0% [83.5%, 2.5%] | 97.3% [95.1%, 2.2%]2 | 96.7% [95.0%, 1.7%] |
1Scaffold statistics are reported for the MaSURca and Canu Hi-C assembly. All other assembly statistics are contig metrics.