Figure 4.

Inhibition by Tubeimoside I (TBMS1) in cells overexpressing wild-type Piezo1. (A) HEK 293T cells were transfected to express exogenous Piezo1 tagged with green fluorescent protein (GFP) (scale bar, 100 μm). (B) Intracellular Ca²⁺ measurements of transfected cells exposed to 5 μM Yoda1 after cells were pretreated with 10 μM TBMS1 or DMSO. Error bars indicate SEM (N = 24 each). (C) Summary of the results in (B) expressed as a percentage of the peak amplitude value after cell pretreatment with DMSO. Error bars represent SEM (n = 5). *P < 0.05. (D) Mean data of the results shown in (B) in which cells were pretreated with the indicated concentrations of TBMS1. Fitting the curve to the Hill equation yields an IC₅₀ of 6.97 μM (n=5). *P < 0.05.