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. 2020 May 25;11:768. doi: 10.3389/fphar.2020.00768

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Copyright © 2020 Liu, Pan, Cheng, Deng, He, Zhang, Ning and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tubeimoside I (TBMS1) inhibits Piezo1 channels in murine liver endothelial cells (MLECs). (A) Extracted murine liver endothelial cells (MLECs) were verified by CD31-labeling of the cell membrane (green) and DAPI-labeling of cell nuclei (blue). Scale bar, 200 μm (B) Intracellular Ca²⁺ responses to 5 μM Yoda1 in MLECs after cell pretreatment for 30 min with 10 μM TBMS1 or DMSO. Error bars indicate SEM (N = 16 each). (C) Summary of the results in (B) expressed as a percentage of the response to Yoda1 in cells pretreated with DMSO only. Error bars indicate SEM (n = 5). *P < 0.05.