Figure 6.

Tubeimoside I (TBMS1) inhibits Yoda1-induced Piezo1 activity in macrophages. (A) End-point PCR products obtained with Piezo1 primers for human (h.) THP-1 cells, and mouse (m.) RAW264.7 cells. (B) Quantitative real-time PCR data for the experiments shown in (A). (C) Western blot of THP-1 cells and RAW264.7 cells with Piezo1 antibody, confirming Piezo1 expression (predicted size, 286 kDa). (D) Quantitative protein data for experiments shown in (C). (E, G) Intracellular Ca²⁺ measurements in THP-1 cells and RAW264.7 cells exposed to 5 μM Yoda1 after cell pretreatment with 10 μM TBMS1 or DMSO. Error bars indicate SEM (N = 24 each). (F, H) Summary of the results shown in (E, G) expressed a percentage of the peak amplitude value relative to the response to 5 μM Yoda1 in cells pretreated with DMSO alone. Error bars represent SEM (n = 5). *P < 0.05.