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High-dose CTX induced imDC-mediated Th cell polarization. (A–D) RT-qPCR analysis of T-bet, GATA-3, RORγt, and Foxp3 mRNA levels in Th cells co-cultured with CTX-treated imDCs for 24 h. (E–I) ELISA detection of IFN-γ, IL-4, IL-17, IL-6, and TGF-β in Th cell/CTX-treated imDC co-culture supernatants. Error bars indicate the SEM. *P < 0.05, compared with the control group. All values represent the mean ± SEM of three independent experiments.
