FIGURE 6.

Effects of variant filtering and live culture storage on mutations detected. (A) Relative frequency of base changes and indels for natural variants found by Gallaher et al. (2015) and the novel variants after UV mutagenesis. (B) Minor allele frequency distribution for all variants and novel variants. (C,D) Average sequencing depth of three biological replicate libraries prepared from dark stored cultures and algae pellets that were frozen at the time of passage. (E,F) Average number of novel mutations detected in libraries from dark stored and frozen tissues. Error bars are standard deviations.