TABLE 3.

MIC values and characteristics of Erava heteroresistance-derived clones.

Strain	MIC (mg/l)		MIC for the effect of EPI (mg/l)				30S ribosome subunit mutation
	Erava	Tige	Erava + CCCP	Erava + PAβN	Tige + CCCP	Tige + PAβN
EF16C62-H1	0.5	0.5	0.06	0.25	≤0.015	0.5	−
EF16C62-H2	0.5	1	0.06	0.5	≤0.015	0.5	−
EF16C62-H3	0.5	1	0.06	0.5	≤0.015	0.5	−
EF16C302-H1	2	8	0.25	2	0.25	≥4	−
EF16C302-H2	1	8	0.25	2	0.12	≥4	−
EF16C302-H3	2	8	0.12	2	0.12	≥4	−
EF16C75-H1	2	8	0.12	2	0.12	≥4	−
EF16C75-H2	2	8	0.12	2	0.12	≥4	−
EF16C75-H3	2	8	0.12	2	0.12	≥4	−
EF16C74-H1	0.5	1	0.06	2	≤0.015	1	−
EF16C74-H2	4	8	0.06	2	≤0.015	≥4	−
EF16C74-H3	4	8	0.06	2	≤0.015	≥4	−

CCCP, carbonyl cyanide m-chlorophenylhydrazone; EPI, efflux pump inhibitor; Erava, Eravacycline; MIC, minimum inhibitory concentration; PaβN, Phe-Arg-β-naphthylamide; Tige, tigecycline.