TABLE 4.

MIC values and mutations in tetracycline binding sites in E. faecalis isolates exhibiting Erava-induced resistance.

Strain	MIC (mg/l)		Mutation in 30S ribosome subunit gene
	Tige	Era	RR1*	RR2*	RR3*	RR4*	S10#
FC1	0.125	0.06	W	W	W	W	W
FC1-1	2	2	W	W	C1265T	W	W
FC1-2	64	16	W	A1461G	C1265T	A1148G	W
					C1191T
FC1-E32	64	16	W	A1461G	C1265T	W	K57Q
					C1191T
FC2	0.125	0.06	W	W	W	W	W
FC2-1	2	2	W	A1461G	W	W	W
FC2-2	32	16	W	A1461G	C1265T	W	K57Q
					C1191T
FC2-E32	32	16	A979T	A1461G	C1265T	G868A	K57Q
			T980A		C1191T	G860A
OG1RF	0.125	0.06	W	W	W	W	W
OG1RF-E4	32	16	G734C	GGACA	C1265T	A231G	53–56 aa del (RATH)
			G1075T	983–987	C1191T	A1148G
				CGGAC
OG1RF-E32	64	16	G734C	C1055G	C1265T	A231G	53–56 aa del (RATH)
			G1075T		C1191T	A1148G

*Four copies of 16S rRNA; ^#30S ribosomal protein S10. aa, amino acid; del, deletion; Era, Eravacycline; MIC, minimum inhibitory concentration; Tige, tigecycline.