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Exogenous administration of Gb3 did not introduce Stx2 susceptibility in DLD-1 cells. DLD-1 cells were incubated with Gb3-containing liposomes or PBS control followed by incubation with Shiga toxin 2 (Stx2) for 24 h. No significant difference in cell metabolism could be seen in PBS-treated or Gb3-liposome treated cells at any of the Stx2 concentrations. Median cell metabolism is denoted by the bar.
