Table 1.
Cell metabolism and protein synthesis in cells exposed to Stx2-positive microvesicles and free toxin.
| Experiments | CHO cells | HeLa cellsa | DLD-1 cellsb | ||||
|---|---|---|---|---|---|---|---|
| CHO-controlb (vector) | Gb3-positive | PPMPb | |||||
| Retro-2.1 | |||||||
| Stx2-pos MVs | Cell metabolism | UCc | ↓↓c | NA | NA | NA | NA |
| Protein synthesis | UCd | ↓↓d,e | ↓e | ↓↓d | UCd | UCd | |
| Free Stx2 | Cell metabolism | UCc | ↓↓c | NA | NA | NA | UCf |
| Protein synthesis | UCd | ↓↓d | NA | ↓↓d | UCd | UCd | |
a
Gb3-positive cells;
b
Gb3-negative cells;
c
Values are presented in Figure 1;
d
Values are presented in Figure 2;
e
Values are presented in Figure 3;
f
Values are presented in Figure 5; ↓, Lowered cell metabolism/viability or protein synthesis; CHO, Chinese hamster ovary; DLD-1, intestinal epithelial cell line; Gb3, Globotriaosylceramide; PPMP, D-threo-1-Phenyl-2-hexadecanoylamino-3-morpholino-1-propanol; Stx2-pos MVs, blood cell-derived Stx2-positive microvesicles; UC, Unchanged; NA, Not assayed.