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. 2020 May 25;10:242. doi: 10.3389/fcimb.2020.00242

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Copyright © 2020 Hajduczki, Danielson, Elias, Bundoc, Scanlan and Berger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of various CAR constructs used in this study. “CD4” moiety is domains 1 and 2 of human CD4, residues 1-208, including the 25 residue signal peptide. “MBL” is the carbohydrate recognition domain of human mannose-binding lectin (residues 214-330). “R5Nt” represents the N-terminal segment (residues 2-26) of human CCR5, with a Cys to Ser substitution at residue 20. “R5Nt(Y/A)” is a control version of R5Nt containing four tyrosine-to-alanine substitutions that eliminate the sulfation targets required for binding to gp120. The recognition domain of the control 139 CAR is an irrelevant scFV. Solid black lines indicate flexible linkers of various lengths. See Supplementary Figure 1 for amino acid sequences.