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. 2020 May 25;10:232. doi: 10.3389/fcimb.2020.00232

Table 1.

Overview of oligonucleotides used as primers in amplification-based methods.

Method Target Sequences (5-3) of oligonucleotidesa used as:
Forward primer Reverse primer
ERG11 sequencing Open reading frame (ORF)
Portion 1 CGAGATAATCATCAACGAACATTC CGTTTAAAACATCCAAAGACCTTA
Portion 2 AATCTGAGGGTTTCCTTGATGGT AAAGACCGCATTGACTACCGAT
Microsatellite typingb Short tandem repeat (STR)
CTT FAM-CCTGGCTTGCAATTTCATTT GCCTCATCGGTGGTGGAATTA
TTG HEX-TTGGAGTAACAAGCGCAGAA GTCGCTTGGACAACTGGTGTA
ACA TAMRA-CAATAGCAGCAATGGAGCAG GTGCTTTTGGTTTGTCCTTGG
GCTTTT FAM-CCAGGTTGGACTATCACTG GGTTTCATTTTGTTGTGAAAA
GTGTTA HEX-CCCTTTCAAAAGAAACAGACA GTTCTATAGATAAAACACACCCCATACA
TGTTGG TAMRA-TGGCGTTAGTATTGGCGTTA GATTGTATCACGCGGGAACTC
a

Sequences of the primer pairs used for ERG11 sequencing or microsatellite typing were described in references of Souza et al. (2015) and Diab-Elschahawi et al. (2012), respectively.

b

One of the amplification primers (i.e., the forward primer) was labeled with either 6-FAM™, JOE™, or TAMRA™.