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. 2020 May 25;11:611. doi: 10.3389/fpls.2020.00611

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Copyright © 2020 Wang, Guo, Wang, Kang, Yang, Shen and Long.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic comparison of wild type and 35S:MtRAV transgenic Arabidopsis thaliana under cold treatment. (A) Expression analysis of cold-related marker genes in 35S:MtRAVs transgenic plants by qRT-PCR: RD29B, COR78, and COR414-TM1. (B) Wild-type and 35S:MtRAV3 transgenic Arabidopsis thaliana were grown on 1/2 MS medium containing 1% agar and grown at 22°C for an additional 18-day. The plates were transferred to a -15°C growth chamber and incubated for 2 h, and then returned to normal growth for 24 h at 22°C. (C) After 24 h normal growth after the -15°C cold treatment, we counted the rate of surviving seedlings. This rate was expressed as a percentage (%). Independent t-tests demonstrated that there was significant difference (^∗P < 0.05).