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. 2020 May 25;11:1037. doi: 10.3389/fmicb.2020.01037

FIGURE 2.

FIGURE 2

Lack of active replication of DWV in T. mercedesae. (A) Detection of DWV VP1 and RdRP in the lysates of individual honey bee pupae and Tropilaelaps mites by western blot. A major 47 kDa band was detected in VP1-posiitive (+) but not negative (−) pupa and mite; however, RdRP (90 and 53 kDa bands) was only present in the VP1-positive pupa. The asterisks represent non-specific proteins cross reacted with anti-RdRP antibody. The size of protein molecular weight marker (MW) is at the left. (B) Frequencies of each codon out of 1,000 codons in DWV and A. mellifera genomes are plotted on the X- and Y-axis, respectively. The red line represents a fitted curve and the Pearson correlation value and P-value are also shown. (C) Frequencies of each codon out of 1,000 codons in DWV and T. mercedesae genomes are plotted on the X- and Y-axis, respectively.