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. 2020 May 25;11:519. doi: 10.3389/fgene.2020.00519

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Copyright © 2020 Molinet, Salinas, Guillamón and Martínez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nucleotide sequence variation for GTR1 gene. (A) Alignment of the promoter and coding regions of the GTR1 gene. The nucleotide sequence of S288c (reference), WE and WA strains were downloaded from the SGRP BLAST server of the University of Toronto (http://www.moseslab.csb.utoronto.ca/sgrp/) and aligned using MUSCLE (http://www.ebi.ac.uk/Tools/msa/muscle/). (B) Scheme of the swap strategy for promoter region, ORF region or both regions. Each promoter and/or ORF was swapped in the opposite genetic background, generating three different combinations. The strain background is shown as an orange (WE) or red (WA) rectangle.