Figure 3.

Biological characterization. (A) In vitro KDM4A enzymatic assay of the compounds at 50 μM. PKF118-310 (5) was used as a positive control at 10 μM. (B–G) Western blot analysis of H3K9me3 (B), H3K36me3 (C), H3K27me2 (D), H3K4me1 (E), H3K4me2 (F), and H3K4me3 (G) in MCF7 breast cancer cells incubated for 24 h with 9ac and 9bc at 25 μM. QC6352 (7) was used as positive controls for 24 h at 10 μM. H4 antibody was used to normalize total proteins loaded. Bands were quantized using ImageJ software. ****p-value ≤ 0.0001, ***p-value ≤ 0.001, **p-value ≤ 0.01, *p-value ≤ 0.05, ^nsp-value > 0.05 vs control cells.