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. 2020 May 25;8:312. doi: 10.3389/fchem.2020.00312

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Copyright © 2020 Souto, Sarno, Nebbioso, Papulino, Álvarez, Lombino, Perricone, Padova, Altucci and de Lera.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell cycle analysis of 9aa, 9ac, and 9bc. HCT-116 cells were treated with 9aa, 9ac, and 9bc for 24 h at 25 μM. SAHA and PKF118-310 (5) were used as positive controls for 24 h at 5 and 10 μM, respectively. Left panel shows the percentage of cell cycle phases and right panel shows the percentage of cell death evaluated by propidium iodide. The analysis was performed using ModFit v3 software. ****p-value ≤ 0.0001, ***p-value ≤ 0.001, **p-value ≤ 0.01, *p-value ≤ 0.05, ^nsp-value > 0.05 vs control cells.