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(A–G) Senescence markers in DNA damage-induced senescence. Coronary VSMCs were treated with 0–25 μg/ml with bleomycin for 3 h and incubated for indicated time-points (n = 2). (H–J) Immuno-fluorescence of HMGB-1 localization (n = 2). Topo II- Topoisomerase 2; 0-25- bleomycin doses in μg per ml; DAPI- 4’,6-diamidino-2-phenylindole; p16, p14, p21- cell cycle inhibitors; IL-1β, IL-6- interleukins; LMNB-1- Lamin B1; HMGB-1- High mobility group box1.
