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. 2020 May 19;24:12–22. doi: 10.1016/j.jot.2020.04.008

Fig. 1.

Fig. 1

Defactinib inhibits H-type vessels in subchondral bone. (A) qRT-PCR analysis of the mRNA expression of FAK in the subchondral bone. n = 5 per group. (B–D) Three-dimensional CT-based microangiography of the medial tibial subchondral bone one month post-surgery (B) with quantification of the vessel volume relative to the tissue volume (VV/TV) (C) and vessel number (VN) (D). n = 10 per group. Scale bar, 500 μm. (E–F) Confocal images (E) and quantification of CD31 and endomucin-positive cells (CD31: green; endomucin: red; merge: yellow). n = 10 per group. Scale bar, 100 μm. Sham = sham controls; ACLT + Veh = vehicle-treated ACLT mice; ACLT + Def = defactinib-treated ACLT mice. ∗p < 0.05 and ∗∗p < 0.01 compared to sham. #p < 0.05 and ##p < 0.01 compared to vehicle.