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. 2020 May 19;24:12–22. doi: 10.1016/j.jot.2020.04.008

Fig. 6.

Fig. 6

Defactinib inhibits MSC-regulated angiogenesis through FAK-Grb2-MAPK-linked VEGF. (A–C) ELISA analysis of the expression of VEGF in CM of MSCs from the different groups (A), subchondral bone marrow (B), and in blood (C). n = 5 per group. (D) Representative images showing tube formation of BMECs on Matrigel. Scale bar: 200 μm. (E–F) Total tube length and total loops were measured to quantify the ability of BMECs to form tubes. n = 5 per group. Sham = CM of MSCs from the sham controls; ACLT + Veh = CM of MSCs from the vehicle-treated ACLT mice; ACLT + Def = CM of MSCs from the defactinib-treated ACLT mice; ACLT + VEGF-anti = addition of VEGF neutralizing antibody in CM of MSCs from the vehicle-treated ACLT mice. ∗p < 0.05 and ∗∗p < 0.01 compared to sham. #p < 0.05, ##p < 0.01 compared to vehicle.