Figure 5.

Changes in mitochondrial network size after acute and chronic exposure to AβOs in hippocampal neurons. (A) Super-resolution images of hippocampal neurons showing the immunoreactivity for the specific primary antibody for TOM 20 (white) in control condition (left panel) and after 24 h of incubation with AβOs (0.5 µM; right panel). (B) Representative 2D and 3D images of mitochondrial population in figure A. (C) The data were analyzed as the percentages of number for volume (> and < 0.3 μm3, C), length (> and < 2 μm, D), and number (E) of mitochondrial population in AβOs treated cells compared with untreated cells. Scale bars: 5 μm. Data are represented as mean ± SEM. *p < 0.05, ***p < 0.001 compared between the control group. Student's t-test followed by unpaired t-test was used for all statistical analyses. TOM20, translocase of outer membrane. (n=3, N=15) (complete inmunocytochemistry for TOM20 with controls are provided on Supplementary Figure 5).