Figure 6.

Changes in SIRT1 and PGC-1α levels after exposure to AβOs. (A) PGC-1α western blot of PC12 lysates control and treated with AβOs (0.5 μM) for 1 and 24 h. (B) SIRT1 western blot of PC12 lysates in the same conditions as A. (C) Quantification of PGC-1α protein levels normalized to β actin levels, and (D) SIRT1 protein levels under similar conditions as C. Data are represented as mean ± SEM. *p < 0.0, **p < 0.01, compared between the control group. One-way ANOVA with the Dunn's multiple comparisons test was used for all statistical analyses. PGC-1, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; SIRT1, silent information regulator 2 homolog 1. (n=5–6/group). A dotted line on the western blot indicates different regions of the same gel (original gel blot are provided on Supplementary Figure 6).