Figure 7.

Immunocytochemistry of SIRT1 and PGC-1α in PC12 cells treated with AβOs. (A) Representative confocal images of PC 12 cells stained with specific primary antibodies for PGC-1α (Green),SIRT1 (red), and DAPI (blue) in control condition (upper panel), and after AβOs treatments (0.5 µM) during different incubation times (1 and 24 h, lower panels). (B) Quantification of the nucleus/cytosol ratio (N/C) for SIRT1 (upper graph) and PGC-1α (lower graph) from images shown in A. (C) Surface plot of a representative cell shown in A, correlating spatial distribution (x,y) with fluorescence intensity (z) for DAPI and PGC-1α in control condition (left panel) and after 24 h of treatment with AβOs (right panel). Scale bars: 20 μm. (D) Manders colocalization coefficient (MCC) values quantify the different levels of colocalization between PGC-1α and SIRT1. Data are represented as mean ± SEM. *p < 0.05, **p < 0.01, compared between the control group. One-way ANOVA with the Dunn's multiple comparisons test was used for all statistical analyses. PGC-1, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; SIRT1, silent information regulator 2 homolog 1. (n=3, N=37).