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. 2020 Aug 10;26(49):11266–11275. doi: 10.1002/chem.202001036

Figure 4.

Figure 4

Steady‐state kinetic parameters for N7‐methylation of probe 6 b catalyzed by Ecm1 and RNMT–RAM. A) Initial rate as a function of probe 6 b concentration in the presence of the SAM cosubstrate and the N7‐MTases: 10 nm Ecm1/50 μm SAM or 20 nm RNMT–RAM/20 μm SAM. B) Initial rate as a function of SAM cosubstrate concentrations in the presence of 2 μm of probe 6 b and the N7‐MTases: Ecm1 (10 nm) and RNMT–RAM (20 nm). Data are the mean±SD of three independent experiments. Calculated kinetic parameters are shown in Table 2.