Figure 7.
Inhibition of N7‐MTase activities of A) Ecm1 and B) RNMT–RAM toward 6 b by the most potent selected substrate analogues and sinefungin. All reaction mixtures contained probe 6 b (2 μm) and either SAM (50 μm), Ecm1 (10 nm) or SAM (20 μm), RNMT–RAM (20 nm) in 50 mM Tris⋅HCl buffer (pH 7.5). The mixtures were incubated at 30 °C in a 96‐well plate reader. The fluorescence measurements were carried out for excitation at 345 nm and emission at 378 nm. Data are the mean±SD of two or three independent experiments. The determined IC50 and Hill slope values are shown in Table 4.