Table 1.
Spectral properties of fluorescently labeled GpppA and GpppG analogues.[a]
|
Compound |
λ max [nm] |
λ max [nm] |
QY [%] |
Q Ecm1 [−] |
|---|---|---|---|---|
|
abbreviation (no.) |
Absorption |
Emission |
|
|
|
Gp3A‐3’‐O‐Py (6 a) |
330, 345 |
378, 398 |
12.3±0.3 |
1.5 |
|
Gp3A‐3’‐O‐Py3 (6 b) |
330, 345 |
378, 398 |
29.8±0.5 |
3.4 |
|
Gp3A‐3’‐O‐Pe (6 c) |
418, 443 |
493 |
99.8±0.9 |
1.0 |
|
Gp3A‐3’‐O‐BDP‐FL (6 d) |
505 |
513 |
54.8±0.1 |
1.0 |
|
Gp3A‐3’‐O‐5‐FAM (6 e) |
498 |
528 |
44.7±0.3 |
1.0 |
|
Gp3A‐3’‐O‐Cy3 (6 f) |
545 |
563 |
49.2±0.1 |
1.1 |
|
Gp3A‐3’‐O‐Cy5 (6 g) |
642 |
660 |
22.6±0.9 |
1.1 |
|
2’‐O/3’‐O‐Py‐Gp3A (7) |
330, 345 |
378, 398 |
11.4±0.3 |
1.1 |
|
Gp3A(N6)‐Py (8) |
330, 345 |
378, 398 |
14.9±0.4 |
1.1 |
|
Gp3G(N1)‐Py (9) |
330, 345 |
378, 398 |
3.9±0.1 |
1.1 |
|
m7Gp3A‐3’‐O‐Py (10 a) |
330, 345 |
378, 398 |
6.57±0.03 |
product[b] |
|
m7Gp3A‐3’‐O‐Py3 (10 b) |
330, 345 |
378, 398 |
4.01±0.02 |
product[b] |
[a] Data are the means±SD of three independent experiments. [b] Obtained by chemical synthesis. Q Ecm1: quenching factor for N7‐methylation reaction by Ecm1. QY: quantum yield.