Table 2.

Steady‐state kinetic parameters for Ecm1 and RNMT–RAM catalysis of N7‐methylation of probe 6 b.

Enzyme	K M	V max	k cat	k cat/K M
	[μm][a]	[mmol min−1  mg−1][a]	[s−1][a]	[s−1⋅μm −1][a]
Ecm1 (10 nm)	22.0±2.4	(3.13±0.24)×10−13	1.64±0.13	0.074±0.010
RNMT–RAM	11.4±2.8	(0.065±0.001)×10−3	0.160±0.025	0.014±0.004
(20 nm)

Conditions: [a] 0–15 μm probe 6 b; 20 μm SAM for RNMT–RAM and 50 μm SAM for Ecm1.