Table 3.

IC₅₀ and p values obtained in the N7‐methylation activity assay (both Ecm1 and RNMT–RAM) for five selected potential inhibitors and GpppA and sinefungin as a control.^[a]

Compound	Ecm1		RNMT–RAM
	IC50 (μm)	p	IC50 (μm)	p
Gp3A	32.6±3.4	−1.0±0.2	>100	n.d.
8‐Me‐Gp3A	5.21±0.68	−1.0±0.1	39.3±7.6	≈−2.0
8‐Ph‐Gp3A	19.4±3.5	−0.8±0.1	>100	n.d.
8‐CF3‐Gp3A	7.33±0.54	−1.9±0.2	104±14	−1.2±0.2
6‐SGDP	32.1±2.1	−2.6±0.6	12.0±1.0	−1.7±0.2
6‐SGp3A	8.93±0.81	−1.3±0.1	7.55±1.00	−1.5±0.3
Sinefungin	0.014±0.002	−1.0±0.1	0.44±0.06	−1.0±0.1

[a] Data are the means±SD of three independent experiments.