Fig. 5.
Activation of EGF‐EGFR‐RAF‐MEK1/2‐ERK1/2 is distinctive of NEMOΔhepa/JNKΔhepa mice. (A) Representative IHC for NOTCH‐1 staining of liver sections of 52‐week‐old NEMOf/f, NEMOΔhepa, and NEMOΔhepa/JNKΔhepa livers, magnification is 20×. (B) Representative IHC for ErbB2 staining of liver sections of the same livers. (C) mRNA expression analysis of notch‐2 (left), erbB2 (center), and egf (right) was quantified by qRT‐PCR of samples taken from NEMOf/f, NEMOΔhepa, and NEMOΔhepa/JNKΔhepa mice killed at 52 weeks. Data are presented as mean ± SEM. *P < 0.05; ****P < 0.0001. (D) Protein expressions of phospho‐β‐catenin, β‐catenin, ErbB2, phospho‐EGFR, phospho‐RAF, phospho‐MEK1/2, phospho‐ERK1/2, and total ERK1/2 from whole‐liver extracts of 52‐week‐old NEMOf/f, JNK1Δhepa/JNK2−/−, NEMOΔhepa, and NEMOΔhepa/JNKΔhepa mice were analyzed by western blot with the indicated antibodies. GAPDH served as loading control. Abbreviation: phospho, phosphorylated.