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Remnant CSF samples were pooled (A–F) and then split into separate 1 ml aliquots. Samples were then processed in parallel to determine run to run variability (A). Overall variability was limited to 12% across pools A–F. Addition of radioimmunoprecipitationassay (RIPA) buffer which contains detergents greatly enhances signal of LRRK2 in CSF (B). ∗p < 0.05.
