TABLE 1.

Epitope mapping data showing the main epitopes of commercially available total LRRK2 monoclonal antibodies.

Antibody Sample	Species	LRRK2 Part	Spot Intensities	Main Epitope	Observations
MJFF1 (c5-8)	Rabbit	C-terminal (970–2527 aa)	High	LDLSANELRDI	None
MJFF2 (c41-2)	Rabbit	C-terminal (970–2527 aa)	High to very high	LSANELRDI	None
MJFF3 (c69-6)	Rabbit	C-terminal (970–2527 aa)	High	LDLSANELRDID	None
MJFF4 (c81-8)	Rabbit	C-terminal (970–2527 aa)	High	SANELRDID	None
MJFF5 (c68-7)	Rabbit	C-terminal (970–2527 aa)	High	LSANELRDI	None
SIG-39840	Mouse	C-terminal (970–2527 aa)	High to very high	FPNEF	Remarkable cross-reaction with peptides with the motifs FAGREEF and DELEF
N241A/34	Mouse	C-terminal (970–2527 aa)	High	EGDLLVNPDQ	None
N231B/34	Mouse	C-terminal (970–2527 aa)	Very weak	LKFPNEFD	Higher intensities with anti-rabbit Ab; cross-reaction with N-terminal motif DEDGHFP
UDD3	Rabbit	N-terminal (1–555 aa)	Very high	HEKI	Short consensus motif; cross-reaction with peptides with motif FFNILVLNEVHEFV
8G10	Mouse	N-terminal (1–555 aa)	High	DEDGHFP	None
N138/6	Mouse	N-terminal (1–555 aa)	Moderate	LNNVQEGKQI	None

Reprinted with permission from the Michael J Fox Foundation for Parkinson’s disease research.