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Dilutions of plasma calibrator to determine linearity.
| Fold dilution | 1st dilution |
2nd dilution (of 1st dilution) |
||
| Vol standard (μL) | Vol PBS (μL) | Vol diluted standard (μL) | Vol PBS (μL) | |
| 20 | 50 | 950 | – | – |
| 40 | 25 | 975 | – | – |
| 50 | 20 | 980 | – | – |
| 66.67 | 15 | 985 | – | – |
| 100 | 10 | 990 | – | – |
| 125 | 8 | 992 | – | – |
| 142.875 | 7 | 993 | – | – |
| 200 | 5 | 995 | – | – |
| 250 | 4 | 996 | – | – |
| 333.33 | 3 | 997 | – | – |
| 400 | 10 | 990 | 250 | 750 |
| 500 | 10 | 990 | 200 | 800 |
| 800 | 10 | 990 | 125 | 875 |
| 1000 | 10 | 990 | 10 | 990 |
| 2000 | 10 | 990 | 50 | 950 |
