Figure 1.

Purification of Full-Length Wild-Type TDP-43 Using 0.2% Sarkosyl

(A) Cell lysis in 50 mM sodium phosphate pH 8.0, 300 mM sodium chloride, 5 mM imidazole, 5 mM dithiothreitol, complete protease inhibitor cocktail, 1 mM phenylmethylsulphonyl fluoride, 50 μg/mL lysozyme, and pure water. SF, soluble fraction; IF, insoluble fraction.

(B) Immobilized metal ion chromatography. SF, soluble fraction; FT, flowthrough; wash fraction (75 mM imidazole), elution with increasing imidazole concentration.

(C) Size exclusion chromatography (SEC) and SDS-PAGE of SEC fractions containing pure wild-type TDP-43.