Fig. 1.
YY1 enhances HIV-1 replication in 293T cell. (A-C) 293T cells were transfected pCMV/Con or pCMV/HA-YY1 with pNL4-3GFP and pDs-Red. Cell lysate were subjected to Western blotting (A) and for quantification of p24 levels, the viral supernatants were analyzed by p24 ELISA, *P < 0.05, Student’s t test, obtained from three independent experiments (B). HIV-1 mRNA was determined by Northern blotting (C). (D-F) 239T cells were transfected with either control siRNA, siTat or siYY1 with pNL4-3GFP and pDs-Red. Western blotting of cell lysate (D), p24 ELISA of viral supernatant (E) and qRT-PCR of Gag RNA (F). The asterisks indicate a significant difference from control, one-way ANOVA with Dunnett’s multiple comparisons test, ***P < 0.0001.