Fig. 2.
YY1 enhances HIV-1 replication in MT-4 cell. (A, B) MT-4 cells were transfected pCDNA3/mCherry or pCDNA3/YY1-mCherry (Y-M). 24 h later, HIV-1NL4-3GFP infected and incubated for three days. (A) p24 ELISA result of viral supernatant. *P < 0.05, Student’s t test, obtained from three independent experiments. (B) Western blotting of cell lysate. (C, D) MT-4 cells were transfected either consiRNA, siTat or siYY1 then HIV-1NL4-3GFP infected and incubated as described above. (C) p24 ELISA result of viral supernatant. (D) Western blotting of cell lysate. The asterisks indicate a significant difference from control, one-way ANOVA with Dunnett’s multiple comparisons test, **P < 0.01, ***P < 0.001. (E-G) MT-4 or MT-4_shYY1 cells were infected HIV-1NL4-3GFP with indicated concentration then incubated for three days. (E) HIV-1 infected cell captured by fluorescent microscope, (F) GFP positive cell were analyzed by FACS, (G) Western blotting. All experiments are performed more three independent experiments.