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. 2020 May 18;23(6):101178. doi: 10.1016/j.isci.2020.101178

Figure 2.

Figure 2

Tumor Education Signatures of TAMs in BrM

(A) Euler diagrams depict the number of unique and shared upregulated (left panel) or downregulated (right panel) DEGs in control versus TAM-MG, or TAM-MDM, or TA-Mono.

(B–D) Volcano plot of control cells versus BrM-associated cells in (B) microglia, (C) MDM, and (D) monocytes. Cutoffs: ± 1 log2 fold change and adjusted p value <0.05.

(E–G) Functional gene annotation of altered cellular pathways in (E) TAM-MG, (F) TAM-MDM, and (G) TA-Mono compared with control cell types from tumor-free animals. Cutoffs: ± 1, log2 fold change and adjusted p value <0.05. All DEGs or top 3,000 DEGs based on adjusted p values were subjected to analysis.

(H–J) Unsupervised clustering of the top 50 DEGs in control versus BrM-associated (small and large stage) TAMs for (H) microglia, (I) MDM, and (J) monocytes. n = 4–5 replicates for each condition.