Table 2.

Validation parameters for RP-HPLC analytical methods, ESK in PBS (pH 7.4) and ESK in rat plasma (Means ± S.D., n = 5).

	Analytical method
	ESK in PBS (pH 7.4)	ESK in rat plasma
Range (μg/ml)	0.625–20	0.2–5
Slope	83.23	0.18
y-intercept	16.89	40.63
r2	0.9999	0.9989
LoD (μg/ml)	0.10	0.05
LoQ (μg/ml)	0.30	0.07